Zavala L, Sobien M, Hayne CK. Biochemical characterizations of Pontocerebellar Hypoplasia linked mutations of Target of EGR1 (TOE1) reveal impacts on thermal stability, ribonuclease activity, and oligomerization. BioRxiv [preprint]. 2025 Sep 18.

Link: https://pubmed.ncbi.nlm.nih.gov/41000718/

Elder,JJH, Papadopoulos, R, Hayne, CK^†, Stanley, RE^†
The making and breaking of tRNAs by ribonucleases, Trends in Genetics, 2024 Apr 18:S0168-9525(24)00069-6.
https://doi.org/10.1016/j.tig.2024.03.007

^†denotes co-corresponding authors

Hayne, CK^†#, Sekulovski, S^#, Hurtig, JE ^#, Stanley, RE^†#, Trowitzsch, S^†#, Hoof Av^†#. New Insights into RNA processing by the eukaryotic tRNA Splicing Endonuclease.JBC 299, 105138, 2023

https://doi.org/10.1016/j.jbc.2023.105138        

^#denotes co-first authors, ^†denotes co-corresponding authors

Hayne, CK^†, Butay JK, Stewart ZD, Krahn JM, Perera L, Williams JG, Petrovitch R, Deterding LJ, Matera AG, Borgnia MJ, Stanley RE^†. Structural basis for pre-tRNA recognition and processing by the human tRNA splicing endonuclease complex. Nat Struct Mol Biol . 2023 30, 824–833

doi: 10.1038/s41594-023-00991-z       

^†denotes co-corresponding authors.  

See highlight in News and Views (click here)

Recognized as a 2023 NIEHS Paper of the Year (click here)!

Hayne CK, Lewis, TA, Stanley RE. Recent insights into the structure, function and regulation of the eukaryotic tRNA splicing endonuclease complex. WIRES RNA. 2022 e1717.

doi: 10.1002/wrna.1717

Pillon MC, Frazier MN, Dillard LB, Williams JG, Kocaman S, Krahn JM, Perera L, Hayne CK, Gordon J, Stewart ZD, Sobhany M, Deterding LJ, Hsu AL, Dandey VP, Borgnia MJ, Stanley RE. Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics. Nat Commun. 2021 Jan 27;12(1):636.

doi: 10.1038/s41467-020-20608-z.

Hayne CK, Schmidt CA, Haque MI, Matera AG, Stanley RE. Reconstitution of the human tRNA splicing endonuclease complex: insight into the regulation of pre-tRNA cleavage. Nucleic Acids Res. 2020 Aug 20;48(14):7609-7622.

doi: 10.1093/nar/gkaa438.

“NAR Breakthrough Article”

Recognized as a 2020 NIEHS Paper of the Year (click here)!

Hayne CK, Yumerefendi H, Cao L, Gauer JW, Lafferty MJ, Kuhlman B, Erie DA, Neher SB. We FRET so You Don’t Have To: New Models of the Lipoprotein Lipase Dimer. Biochemistry. 2018 Jan 16;57(2):241-254. doi: 10.1021/acs.biochem.7b01009. Epub 2018 Jan 5.

Hayne CK, Lafferty MJ, Eglinger BJ, Kane JP, Neher SB. Biochemical Analysis of the Lipoprotein Lipase Truncation Variant, LPLS447X, Reveals Increased Lipoprotein Uptake. Biochemistry. 2017 Jan 24;56(3):525-533. doi: 10.1021/acs.biochem.6b00945. Epub 2017 Jan 9.

Garrett CK, Broadwell LJ, Hayne CK, Neher SB. Modulation of the Activity of Mycobacterium tuberculosis LipY by Its PE Domain. PLoS One. 2015;10(8):e0135447. doi: 10.1371/journal.pone.0135447. eCollection 2015.